Proteus is a Webtool, database, and method to propose mutations for proteins used in industrial applications. Proteus uses the hypothesis of mutation transference of residue pairs in contact detected in PDB to suggest mutations for a target protein.
Barroso, J.M., Mariano, D., Dias, S.R. et al. Proteus: An algorithm for proposing stabilizing mutation pairs based on interactions observed in known protein 3D structures. BMC Bioinformatics 21, 275 (2020). https://doi.org/10.1186/s12859-020-03575-6
Select a PDB file and a chain to start a new project. Optionally, you can insert an amino acid residue to limit the
region for suggesting mutations (and accelerate the running).
If you want to test Proteus, you can download this
PDB example (2LZM).
Type A in the second field, S44 in the third field,
and insert your email address.
Proteus will take 1 hour to process your project. If you do not want to wait,
click here to see
the final result.